Mechanisms of Anthracycline-Enhanced Reactive Oxygen Metabolism in Tumor Cells

Table 8

Effect of anthracycline antibiotics on oxygen consumption by Ehrlich tumor cells. Oxygen consumption by Ehrlich cells was examined at 37°C as described in Materials and Methods; the total 3 ml volume contained tumor cells and the final KCN concentration, where used, was 2 mM.

Reaction system	Oxygen consumption (nmol O2/min/ cells)
	+KCN	-KCN
Control	^a
+BCNU (100 μg/ml)
+Glucose (10 mM)
+Antimycin A (10 μg/ml) replacing KCN
-Cells	N.D.^b
Doxorubicin (90 μM)	^c
Doxorubicin (200 μM)	^c
Doxorubicin (400 μM)	^c
+BCNU (100 μg/ml)	^d	—
+Adenosine (1 mM)		—
+Glucose (10 mM)	^d
+Antimycin A (10 μg/ml) replacing KCN	^c	—
-Cells	N.D.	—
Doxorubicin (1 mM)	^c	—
Daunorubicin (400 μM)	^c	—
5-Iminodaunorubicin (400 μM)	06	—

^a of 3 to 15 experiments; ^bN.D. is not detectable; ^csignificantly different from control, at ; ^dsignificantly different from samples containing doxorubicin alone, at .