Research Article

Indigo Naturalis Suppresses Colonic Oxidative Stress and Th1/Th17 Responses of DSS-Induced Colitis in Mice

Figure 4

Effects of QD on mRNA and protein expression of Th1/Th17-related cytokines and transcription factors in colon tissues of mice with DSS-induced colitis: (a) mRNA expression of IFN-γ, T-bet, IL-17A, and RORγt; (b) protein expression of IFN-γ, IL-17A/F, TNF-α, and IL-1β. Colitis was induced in all groups except the control group. QD and SASP were administered to mice from days 6 to 12. On day 13, the mice were sacrificed and mRNA expressions of IFN-γ, T-bet, IL-17A, and RORγt in the colon tissues were measured using RT-PCR. Protein expressions of IFN-γ, IL-17A/F, TNF-α, and IL-1β in the colon tissues were measured using ELISA. Data were expressed as (). #, ##, and ###, compared with the control group; and , compared with the DSS model group.
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