Hsp90 Inhibitor SNX-2112 Enhances TRAIL-Induced Apoptosis of Human Cervical Cancer Cells via the ROS-Mediated JNK-p53-Autophagy-DR5 Pathway

Figure 1

SNX-2112 enhances TRAIL-induced cytotoxicity in human cervical cancer cells. (a) Chemical structure of SNX-2112. (b) Cervical cancer cell lines, HeLa, SiHa, and Caski, were treated with TRAIL at different concentrations (0, 31.25, 62.5, 125, 250, 500, and 1000 ng/mL) for 48 h. Cell viability was assessed by MTT assay. (c) Cervical cancer cell lines, HeLa and SiHa, were treated with SNX-2112 at different concentrations (0, 31.25, 62.5, 125, 250, 500, and 1000 nM) for 48 h. Cell viability was assessed by MTT assay. (d) HeLa or (e) SiHa cells were treated with either TRAIL (200 ng/mL) or SNX-2112 (125, 250 nM) or in combination for 48 h. Cell viability was assessed by MTT assay. (f-g) Fitting curves of CIs for TRAIL and SNX-2112 combination in SiHa and HeLa cell lines plotted and calculated using CompuSyn software. Data are represented as . Error bars represent SD from three separate experiments. and compared with the control group.

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