Nickel Enhances Zinc-Induced Neuronal Cell Death by Priming the Endoplasmic Reticulum Stress Response

Figure 3

GT1-7 cells (6-well culture plates at a density of cells per well) were incubated with NiCl₂ (Ni, 40 μM) in the absence (control) or presence of ZnCl₂ (Zn, 25 μM) for 7 h. Whole-cell extracts were analyzed by immunoblotting with an antibody against CHOP or actin (a). Band intensity was determined using ImageJ software (b). GT1-7 cells (96-well culture plates at a density of cells per well) were pretreated with the indicated concentrations (μM) of TUDCA just before Ni²⁺/Zn²⁺ treatment. Next, GT1-7 cells were incubated in the absence (control) or presence of NiCl₂ (20 or 40 μM) and ZnCl₂ (25 μM) for 24 h (c, d). GT1-7 cells (96-well culture plates at a density of cells per well) were treated with the indicated concentrations of TUDCA for 24 h (e). Cell viability was determined using CellTiter-Glo® 2.0 (c–e). Values represent values are described in the figure when : (black: control, red: vs. ZnCl₂ alone (b)) or (black: vs. control, red: vs. Zn(25)/Ni(20 or 40) (c, d)).

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