The Specific Inhibition of SOD1 Selectively Promotes Apoptosis of Cancer Cells via Regulation of the ROS Signaling Network

Figure 1

The specific SOD1 inhibition alters gene expression of numerous key signaling proteins in HeLa cells. (a) LD100, a specific SOD1 inhibitor. (b) The cluster analysis of DEGs. Based on normalized log₁₀(FPKM+1), the heat map is drawn using red and blue for high-expressed genes and low-expressed genes, respectively. (c) A Venn diagram showing the overlaps between the differentially expressed genes (DEGs) in control, LD100-treated, and SOD1 knockdown HeLa cells. (d, e) Enriched KEGG pathway scatterplots of DEGs in the SOD1 inhibition group (d) and SOD1 knockdown group (e). The scatterplot shows the top 20 signaling pathways with a high level of statistical significance. (f) The cluster analysis of DEGs in signaling pathways of a cancer cell. Based on normalized log₁₀(FPKM+1), the heat map is drawn using yellow and red for high-expressed genes and low-expressed genes, respectively. (g) The cluster analysis of DEGs in MAPK, PI3K-Akt, NF-κB, and cell cycle signaling pathways. Based on normalized log₁₀(FPKM+1), these heat maps are drawn using yellow and red for high-expressed genes and low-expressed genes, respectively.

(a)

(b)

(c)

(d)

(e)

(f)

(g)