Research Article

The Role of Nrf2 Signaling Pathway in Eucommia ulmoides Flavones Regulating Oxidative Stress in the Intestine of Piglets

Figure 4

Intracellular glutathione (GSH) and oxidized glutathione (GSSG) concentrations. IPEC-J2 cells were treated with DMEM-H medium (control) or with 70 μM paraquat (PQ) or 1.9 μM specific Nrf2 inhibitor (ML385) for 12 h. The concentrations of GSH and GSSG were determined using a GSH and GSSG assay kit from Beyotime Institute of Biotechnology. The ratio of GSSG to GSH was calculated. Data expressed as ; independent experiments. Different letters (A, B, and C) indicate significant differences ().