Oxidative Medicine and Cellular Longevity / 2019 / Article / Fig 7

Research Article

Endogenous CSE/Hydrogen Sulfide System Regulates the Effects of Glucocorticoids and Insulin on Muscle Protein Synthesis

Figure 7

Insulin increased protein synthesis, CSE expression, and the activity of the mTOR/p70S6K signalling pathway. Different doses of insulin (0, 1, 5, or 10 μg/mL) were administered to the myoblasts for 6 h. All cells were then incubated with puromycin (10 μM) for 30 min to evaluate protein synthesis rates. Cell lysates were immunoblotted with the following specific antibodies: (a) puromycin, (b) CSE and CBS, (c) mTOR or p-mTOR (Ser 2448), and (d) p70S6K or p-p70S6K (Thr389). The data are presented as the (); ; .

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