The Impact of Hypertension and Metabolic Syndrome on Nitrosative Stress and Glutathione Metabolism in Patients with Morbid Obesity

Table 2

Area under the curve (AUC) of nitrosative stress biomarkers and glutathione between patients with morbid obesity (OB) and morbid obesity and hypertension (OB+HYP); patients with morbid obesity (OB) and morbid obesity and metabolic syndrome (OB+MS) as well as patients with morbid obesity and hypertension (OB+HYP) and morbid obesity and metabolic syndrome (OB+MS); reduced glutathione (GSH), oxidized glutathione (GSSG), myeloperoxidase (MPO) nitric oxide (NO).

	OB vs. OB+HYP
	AUC	95% CI	value	Cutoff	Sensitivity %	95% CI	Specificity %	95% CI
MPO	0.8021	0.6770 to 0.9271	0.0008	>0.1358	75	50.50% to 89.82%	76.67	59.07% to 88.21%
NO	0.6583	0.4810 to 0.8357	0.0796	< 10.31	75	50.50% to 89.82%	73.33	55.55% to 85.82%
S-nitrosothiols	0.6375	0.4573 to 0.8177	0.128	<20.24	62.5	38.64% to 81.52%	63.33	45.51% to 78.13%
Peroxynitrite	0.9292	0.8568 to 1.000	<0.0001	>5.705	87.5	63.98% to 97.78%	90	74.38% to 96.54%
Nitrotyrosine	0.6167	0.4442 to 0.7891	0.1965	<5.572	56.25	33.18% to 76.90%	60	42.32% to 75.41%
Total glutathione	0.619	0.4355 to 0.8024	0.1853	>46.46	62.5	38.64% to 81.52%	67.74	50.14% to 81.43%
GSSG	0.7036	0.5191 to 0.8882	0.0234	>31.56	68.75	44.40% to 85.84%	64.52	46.95% to 78.88%
GSH	0.6573	0.5000 to 0.8145	0.0799	<12.39	62.5	38.64% to 81.52%	64.52	46.95% to 78.88%
Redox potential	0.7333	0.5848 to 0.8819	0.011	<3.618	66.67	41.71% to 84.82%	74.19	56.75% to 86.30%
	OB vs. OB+MS
	AUC	95% CI	value	Cutoff	Sensitivity %	95% CI	Specificity %	95% CI
MPO	0.8563	0.7481 to 0.9644	<0.0001	>0.1705	75	50.50% to 89.82%	76.67	59.07% to 88.21%
NO	0.7833	0.6525 to 0.9141	0.0017	>15.72	75	50.50% to 89.82%	73.33	55.55% to 85.82%
S-nitrosothiols	0.8458	0.7109 to 0.9807	0.0001	>24.02	81.25	56.99% to 93.41%	83.33	66.44% to 92.66%
Peroxynitrite	0.9125	0.8317 to 0.9933	<0.0001	>5.481	81.25	56.99% to 93.41%	83.33	66.44% to 92.66%
Nitrotyrosine	0.8125	0.6742 to 0.9508	0.0005	>7.339	75	50.50% to 89.82%	76.67	59.07% to 88.21%
Total glutathione	0.5484	0.3708 to 0.7260	0.59	>40.60	43.75	23.10% to 66.82%	45.16	29.16% to 62.23%
GSSG	0.5948	0.4208 to 0.7687	0.2913	>30.06	62.5	38.64% to 81.52%	61.29	43.82% to 76.27%
GSH	0.5948	0.4232 to 0.7663	0.2913	<12.78	62.5	38.64% to 81.52%	61.29	43.82% to 76.27%
Redox potential	0.6215	0.4418 to 0.8012	0.1855	<6.495	60	35.75% to 80.18%	58.06	40.77% to 73.58%
	OB+HYP vs. OB+MS
	AUC	95% CI	P value	Cutoff	Sensitivity %	95% CI	Specificity %	95% CI
MPO	0.6523	0.4540 to 0.8507	0.1416	>0.1743	62.5	38.64% to 81.52%	62.5	38.64% to 81.52%
NO	0.8594	0.7158 to 1.000	0.0005	>14.11	81.25	56.99% to 93.41%	81.25	56.99% to 93.41%
S-nitrosothiols	0.9141	0.8106 to 1.000	<0.0001	>22.41	81.25	56.99% to 93.41%	81.25	56.99% to 93.41%
Peroxynitrite	0.5391	0.3292 to 0.7489	0.7063	>7.290	56.25	33.18% to 76.90%	56.25	33.18% to 76.90%
Nitrotyrosine	0.8945	0.7781 to 1.000	0.0001	>6.945	81.25	56.99% to 93.41%	81.25	56.99% to 93.41%
Total glutathione	0.5703	0.3595 to 0.7811	0.4975	<44.41	62.5	38.64% to 81.52%	62.5	38.64% to 81.52%
GSSG	0.6367	0.4351 to 0.8384	0.1871	<32.50	62.5	38.64% to 81.52%	62.5	38.64% to 81.52%
GSH	0.5586	0.3551 to 0.7620	0.5718	>11.52	56.25	33.18% to 76.90%	56.25	33.18% to 76.90%
Redox potential	0.5778	0.3630 to 0.7926	0.4679	>3.081	53.33	30.12% to 75.19%	53.33	30.12% to 75.19%