Inhibitory Effect of Ursolic Acid on Ultraviolet B Radiation-Induced Oxidative Stress and Proinflammatory Response-Mediated Senescence in Human Skin Dermal Fibroblasts

Figure 7

Fluorescent microscopy imaged of acridine orange/ethidium bromide staining to observed the apoptotic morphological changes in HDFs treated with UA, UVB radiation, and UA+UVB radiation; (a, b) green fluorescence of acridine orange accumulation indicating that apoptosis was not induced by UA 20 μM treated cells; (c) red staining of ethidium bromide indicates severe apoptosis induction in HDFs by UVB 40 mJ/cm²; (d) red and green fluorescent staining showed moderate apoptosis by UA 10 μM+=UVB 40 mJ/cm²; (e, f) strong green staining indicated that apoptosis reduced the treatment of UA 20 μM+UVB 40 mJ/cm² and UA 40 μM+UVB 40 mJ/cm². Data analyses are shown in the graph below images. All images were captured at 40x magnification. Values are given as of six experiments in each group; statistical analysis was performed by one-way ANOVA followed by DMRT at , , and compared to the nonirradiated control. values not sharing a common marking (, , and ) differ significantly (DMRT).