Astragalus membranaceus Injection Suppresses Production of Interleukin-6 by Activating Autophagy through the AMPK-mTOR Pathway in Lipopolysaccharide-Stimulated Macrophages

Figure 8

Activation of autophagy can reduce the expression level of IL-6. (a) Western blot analysis detecting the effects of Rap and 3-MA on the level of IL-6 in ANA-1 cells. β-Actin was used as the control. (b) Western blot analysis detecting the effects of AM and LPS on ATG5, LC3, and IL-6 proteins in ANA-1 cells before and after knocking down ATG5. β-Actin was used as the control. Data represent (). Asterisks () indicate significant differences compared with the control group (, ). Number signs (#) indicate significant differences compared with the LPS group (^#, ^##).

(a)

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