Research Article

Metformin Protects ARPE-19 Cells from Glyoxal-Induced Oxidative Stress

Figure 3

Metformin reduced apoptosis of ARPE-19 cells induced by glyoxal. Glyoxal-treated ARPE-19 cells were treated with or without metformin (0.1 mM) for 24 h. The apoptotic cells were detected with a TUNEL assay. TUNEL-positive cells were stained in green and nuclei were counterstained in blue. The slides were observed under a fluorescent microscope (a) and quantified (b). Data are expressed as the (, versus glyoxal group). NC: negative control; PC: positive control; N: normal control; G: glyoxal-treated group; and G+M: glyoxal and metformin cotreated group. Scale bar, 20 μm.
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