Stanniocalcin-1 Alleviates Contrast-Induced Acute Kidney Injury by Regulating Mitochondrial Quality Control via the Nrf2 Pathway

Figure 2

rhSTC1 treatment attenuates inflammation injury and mitochondrial ROS generation in HK-2 cells. HK-2 cells were treated with iohexol (200 mg I/ml) with or without rhSTC1 at different concentrations (50 ng/ml, 100 ng/ml) at indicated time courses, respectively. (a) Western blot analysis of the expression of HMGB1 and NLRP3 (). (b, c) Quantification of average Western blot band intensities. (d–f) Representative images of mitochondrial ROS generation (d) and mitochondrial DNA release (e) (). DAPI staining was performed to label nuclear (blue). Mitochondrial ROS was labeled by MitoSOX (red). Mitochondrial DNA was labeled by the indicated antibody (green). Fluorescence images were taken by a LSM780 confocal microscope (magnification, ×630). Values were presented as . , compared with the control group. ^#, compared with the iohexol group.

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