Stanniocalcin-1 Alleviates Contrast-Induced Acute Kidney Injury by Regulating Mitochondrial Quality Control via the Nrf2 Pathway

Figure 9

STC1 plays an antiapoptotic role partly by upregulating the expression of Nrf2. (a) HK-2 cells were then exposed to iohexol (200 mg iodine/ml) for 4 hours with or without rhSTC1 at different concentrations (50 ng/ml, 100 ng/ml). Western blot analysis of Nrf2, Keap1, and HO-1 in the whole cells (). (b) HK-2 cells were then exposed to iohexol (200 mg iodine/ml) for 4 hours with or without rhSTC1 (50 ng/ml) or TBHQ (40 ng/ml). Western blot analysis of the expression of cleaved caspase 3 and HO-1 in the whole cells (). (c) HK-2 cells were firstly transfected with STC1-siRNA, or control siRNA, and 24 hours later, these cells were treated with iohexol (200 mg iodine/ml) for 4 hours with or without rhSTC1 (50 ng/ml) or TBHQ (40 ng/ml). Western blot analysis of the expression of cleaved caspase 3 and HO-1 in the whole cells (). (d) Representative images. HK-2 cells were exposed to iohexol (200 mg iodine/ml) for 4 hours and then treated with Nrf2-FITC (green) to label the distribution of Nrf2. Fluorescence images were taken by a LSM780 confocal microscope (magnification, ×630). (e, f, h, j, l, and m) Quantification of average Western blot band intensities. (g) Semiquantitative fluorescence analysis. (i) Expression of STC1 mRNA level. Scale bar, 10 μM. Values were presented as . , compared with the control group. ^#, compared with the iohexol group. ^▲, compared with the STC1-siRNA treatment group.

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