Inhibition of ERRα Aggravates Sepsis-Induced Acute Lung Injury in Rats via Provoking Inflammation and Oxidative Stress
Knockdown of ERRα deteriorated LPS-induced endothelial hyperpermeability in PMVECs. (a) Representative images were taken to observe the cell morphology of normal rat PMVECs (upper left panel) and the expression level of green fluorescent protein GFP (upper right panel) observed under an inverted fluorescence microscope after 72 hours of infection. . The expression levels of ERRα were detected by Western blot (lower panel). (b) Comparison of endothelial permeability in the four groups at different time points. (c) Representative blots of ZO-1, Occludin, JAM-A, and VE-cadherin in the four groups (upper panel); quantitative results of ZO-1, Occludin, JAM-A, and VE-cadherin (lower panel). The values are expressed as .a compared with the Ctr and shERRα group; b compared with the LPS group.
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