Research Article
Redox States of Protein Cysteines in Pathways of Protein Turnover and Cytoskeleton Dynamics Are Changed with Aging and Reversed by Slc7a11 Restoration in Mouse Lung Fibroblasts
Figure 2
Overexpression of Slc7a11 restored age-dependent oxidation of extracellular Eh(Cys/CySS) in old fibroblasts. (a) Slc7a11 mRNA expression and (b) extracellular Eh(Cys/CySS) were measured as described in materials and methods. Primary lung fibroblasts from one young and one old mouse were isolated, and old fibroblasts were transfected with Slc7a11-overexpressing plasmid (pSlc7a11). For each experimental condition, 3 replicate wells of a 6-well plate were seeded with fibroblasts, and 24 h later, the media were changed to 0 mV redox media for 4 h. Data are expressed as of 3 independent replicates. indicates by 1-way ANOVA with Tukey’s post hoc test adjusted for multiple comparisons.
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