Redox States of Protein Cysteines in Pathways of Protein Turnover and Cytoskeleton Dynamics Are Changed with Aging and Reversed by Slc7a11 Restoration in Mouse Lung Fibroblasts

Figure 4

Volcano plots showing peptides with age-dependent and Slc7a11-reversible Cys redox states. Each dot represents one Cys-containing peptide. (a) Age-dependent peptide redox states are depicted as the fold-change (FC) in old relative to young cells on the -axis and value on the -axis. The 151 peptides above the dashed line (shaded area) were considered to contain cysteines whose redox states changed significantly in response to aging (age-dependent Cys redox state). (b) The effects of Slc7a11 overexpression on the age-dependent peptides from panel A are shown. Old cells transfected with Slc7a11 were compared to young cells. The peptides containing age-dependent Cys redox states in panel A were selected and plotted. Peptides below the dashed line (shaded area) were considered to contain Slc7a11-reversible Cys redox states. Altogether, 104 proteins contained 116 cysteines whose redox states were reversed by Slc7a11 overexpression. Three replicates of young, old, and old Slc7a11-overexpressing fibroblasts were used to calculate the values.

(a)

(b)