Redox States of Protein Cysteines in Pathways of Protein Turnover and Cytoskeleton Dynamics Are Changed with Aging and Reversed by Slc7a11 Restoration in Mouse Lung Fibroblasts

Figure 6

Protein-protein interaction (PPI) network showed proteins with age-dependent Slc7a11-responsive cysteines were clustered in IPA-predicted pathways. The upper left cluster was associated with cytoskeleton and cellular interaction, including actin signaling and integrin-linked kinase (ILK) signaling. The upper right cluster was associated with protein translation, including eukaryotic translation initiation factor 2 (EIF2) signaling. The lower cluster was related to protein ubiquitination and degradation. Proteins that contained age-dependent oxidized, reduced, or both oxidized and reduced cysteines were shown in round, triangular, or rectangular boxes, respectively.