The Antiaging Activity of Ergothioneine in UVA-Irradiated Human Dermal Fibroblasts via the Inhibition of the AP-1 Pathway and the Activation of Nrf2-Mediated Antioxidant Genes

<div>EGT upregulated Nrf2 nuclear translocation in HSF cells. (a, b) EGT upregulated Nrf2 protein levels. HSF cells were treated with different concentrations of EGT (0.125-0.5 <i>μ</i>M) for 1 h or 0.5 <i>μ</i>M EGT for 1-4 h. Western blot results indicated the effect of EGT on total Nrf2 protein levels in whole cells. <i>β</i>-Actin was used as the internal control. Changes in Nrf2 bands were analyzed by densitometry. (c) EGT increased the nuclear translocation of endogenous Nrf2. HSF cells were treated with 0.5 <i>μ</i>M EGT for 1-4 h. Western blot results indicated the effect of EGT on Nrf2 protein levels in the nucleus and cytoplasm. For internal controls, histone and <i>β</i>-actin proteins were used.</div>

Oxidative Medicine and Cellular Longevity

fig4

Figure 4

Figure 4: The Antiaging Activity of Ergothioneine in UVA-Irradiated Human Dermal Fibroblasts via the Inhibition of the AP-1 Pathway and the Activation of Nrf2-Mediated Antioxidant Genes 

Figure 4 | The Antiaging Activity of Ergothioneine in UVA-Irradiated Human Dermal Fibroblasts via the Inhibition of the AP-1 Pathway and the Activation of Nrf2-Mediated Antioxidant Genes 