The Antiaging Activity of Ergothioneine in UVA-Irradiated Human Dermal Fibroblasts via the Inhibition of the AP-1 Pathway and the Activation of Nrf2-Mediated Antioxidant Genes

Figure 8

Nrf2 knockdown attenuated the antioxidant protein expression-mediated protective effects of EGT in HSF cells. Cells were transfected with a specific siRNA against Nrf2 or a nonsilencing control. Following the transfection, cells were incubated with EGT (0.5 μM) for 1 or 6 h. The protein levels of Nrf2 (1 h) or HO-1 and γ-GCLC (6 h) in control siRNA-transfected and siNRf2-transfected cells were measured by Western blot analysis.