Resveratrol Inhibits Ischemia-Induced Myocardial Senescence Signals and NLRP3 Inflammasome Activation

Figure 6

RSV resisted NRCM senescence induced by hypoxia. (a) SA-β-Gal staining of NRCMs. (b) Percentage of SA-β-Gal-staining-positive cells. (c) Immunofluorescence staining of p16 and p19 in NRCMs. (d) Percentage of p16- and p19-positive cells. (e) Immunofluorescence staining of p53. (f) Calculated percentage of p53-positive nuclei. (g) Representative blots and (h) relative expression levels of Sirt1, p53, AC-p53, p16, and p19 in NRCMs. (h) Relative expression levels of Sirt1, p53, AC-p53, p16, and p19 in NRCMs. (i) Representative blots and (j) relative expression levels of Sirt1, p53, AC-p53, and p19 in NRCMs with or without EX527. Each experiment was repeated three times independently and set up three repeats for each time of experiment. NRCMs were harvested after 64 h or hypoxia or RSV treatment. Data were presented as the . Two-way ANOVA was performed for significance examination , the HypO2 group vs. the CON or RSV group; #, the HypO2+RSV group vs. the HypO2 group.

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