Resveratrol Inhibits Ischemia-Induced Myocardial Senescence Signals and NLRP3 Inflammasome Activation

Figure 7

RSV protected against NRCM apoptosis induced by hypoxia. (a) TUNEL staining showing NRCM apoptosis induced by hypoxia. (b) Calculated percentage of TUNEL-positive nuclei. (c–e) Representative blots and relative expression levels of c-caspase 3 and Bax in NRCMs. Each experiment was repeated three times independently. (f) Adenovirus-mediated p53 overexpression contributed to NRCM apoptosis. RSV treatment could not prevent against hypoxia/reoxygenation induced NRCM apoptosis after p53 overexpression. (g) Calculated the ratio of apoptotic NRCM in different groups. (h) Adenovirus-mediated p53 overexpression contributed to Bax overexpression, which could not be inhibited after RSV treatment. (i) Relative quantitative expression of p53 and Bax. Cardiomyocytes were exposed to 12 h of hypoxia followed by 24 h of reoxygenation. Data are presented as the . among indicated groups.

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