Amyloid Beta-Peptide Increases BACE1 Translation through the Phosphorylation of the Eukaryotic Initiation Factor-2α

Figure 5

Subtoxic H₂O₂ increases BACE1 translation. Human neuroblastoma cells were challenged with subtoxic concentrations of H₂O₂ (10 μM) for 24 h. BACE1, p-eIF2α, and eIF2α expressions were studied by (a) western blot and (d) immunofluorescence. The bands corresponding to (b) BACE1/tubulin and (c) p-eIF2α/eIF2α were quantified and expressed as arbitrary units (A.U.). Data are of 3-9 independent experiments. vs. control cells by Student’s -test. BACE1 expression by immunofluorescence analysis was quantified (e) and expressed as fluorescence intensity (F.I.). Data are of 3 independent experiments. vs. control cells by Student -test. Bars represent 5 μm.

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