Oxidative Stress in Alzheimer’s Disease: In Vitro Therapeutic Effect of Amniotic Fluid Stem Cells Extracellular Vesicles

Figure 3

Effect of AFSC-EV supplementation on apoptotic and autophagic pathways of AD primary neurons in vitro. (a) Representative graph showing MTT viability of WT and FAD cells treated or not with AFSC-EV. value < 0.0001. (b) Representative graph showing the percentage of positive cells to Annexin V/Propidium Iodide assay, as described in the 2.12 section. value < 0.05; value < 0.01. (c) Western blot analysis of total lysate of WT, FAD cells treated or not with AFSC-EV, then revealed with anti-Bcl-2, anti-PARP, anti-pAkt, anti-LC3β, and anti-SIRT1 antibodies. The graphs represent the of densitometric analysis of 3 experiments, normalized to actin values. value < 0.05; value < 0.01; value < 0.001.

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