Anticancer Activity of Liquid Treated with Microwave Plasma-Generated Gas through Macrophage Activation

Figure 3

Activation of Raw 264.7 at 24 h elapsed from PGNO-media treatment in various dilution ratios: (a) phase contrast bright field images showing morphological changes. Scale bars are 20 μm. (b) Flow cytometry plot of forward scattering (FSC) and side scattering (SSC) showing morphological changes (). (c, d) Mean values of FSC and SSC ratios, respectively. (e) Phase contrast bright field images of IL-4-pretreated cells for 24 h. Cells were then incubated in LPS and PGNO-media dilutions for 24 h. (f) MFI of DAFFMDA-stained cells that were treated the same as (e) (). IL-4-pretreated macrophages were incubated in PGNO-media or 50 μM SNAP with or without cPTIO for 24 h. ; ^†.

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