Research Article
Anticancer Activity of Liquid Treated with Microwave Plasma-Generated Gas through Macrophage Activation
Figure 4
Depolarization of IL-4-pretreated Raw 264.7 macrophages at 24 h elapsed from PGNO-media treatment in various dilution ratios: (a, b) transcriptional level changes of macrophage M1 polarization-related genes, (a) including iNOS, IL6, and TNF-α, and (b) M2 polarization-related genes, including ARG, IL10, TGF-β, CCL17, EGF, and MMP9. (c) Protein level changes of iNOS. All tests were repeated three times. (; †). (d) Flow cytometry analysis of iNOS, CD86, and CD163 proteins.
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