Research Article
lncRNA OTUD6B-AS1 Exacerbates As2O3-Induced Oxidative Damage in Bladder Cancer via miR-6734-5p-Mediated Functional Inhibition of IDH2
Figure 5
miR-6734-5p exacerbates lncRNA OTUD6B-AS1-induced oxidative damage. (a–d) ROS, MDA, SOD, and GSH-Px production in bladder cancer cells following lncRNA OTUD6B-AS1 overexpression or transfection with mimics. (e–g) Nrf2 expression in cytoplasm and nucleus following lncRNA OTUD6B-AS1 overexpression or transfection with mimics. (h) Apoptosis determined by flow cytometry following lncRNA OTUD6B-AS1 overexpression or transfection of mimics. (i) Statistical analysis of flow cytometry apoptosis data. (j) Apoptosis was determined by TUNEL staining following lncRNA OTUD6B-AS1 overexpression or transfection of mimics. (k) Statistical analysis of cells positive for TUNEL staining. (l) Cell viability was determined by CCK-8 assay following lncRNA OTUD6B-AS1 overexpression or transfection of mimics. , vs. control cells; #, ## vs. cells overexpressing lncRNA OTUD6B-AS1; &, && vs. cells transfected with mimics.
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