Amarogentin from Gentiana rigescens Franch Exhibits Antiaging and Neuroprotective Effects through Antioxidative Stress
Neuroprotection effect of amarogentin on the H2O2-induced oxidative damage in the PC12 cells. (a) Relative viability of the PC12 cells after treatment with H2O2 at different concentrations for 1 h. With an increase in H2O2 concentration, survival rates decreased significantly compared with the control group. (b) Neuronal protection of amarogentin at 1, 3, and 10 μM with or without the H2O2 stimulation. (c) Effect of amarogentin with or without H2O2-induced on ROS production in PC12 cells as detected by fluorescence microplate reader. (d) Photomicrographs of PC12 cells stained with DCFH-DA under a fluorescence microscope. Control (A), RES (10 μM) (B), amarogentin (1 μM) (C), amarogentin (3 μM) (D), amarogentin (10 μM) (E), H2O2-treated control (F), H2O2+RES (10 μM) (G), H2O2+amarogentin (1 μM) (H), H2O2+amarogentin (3 μM) (I), and H2O2+amarogentin (10 μM) (J); scale bar, 20 μm. The levels of MDA content (e), total SOD activities (f), SOD2 activities (g), and SOD1 activities (h) were measured by corresponding assay kits. The PC12 cells were pretreated with RES (10 μM) and different concentrations of amarogentin (1, 3, and 10 μM) for 24 h and then subjected to H2O2 (0.9 mM) for 1 h or treated with RES (10 μM) and amarogentin (1, 3, and 10 μM) alone for 24 h. Experiments were repeated thrice, and the data were presented as .,, and compared with the control group; ,, compared with the H2O2-treated group (grey color bar).
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