Fucoidan Inhibits NLRP3 Inflammasome Activation by Enhancing p62/SQSTM1-Dependent Selective Autophagy to Alleviate Atherosclerosis
Fucoidan enhances the impaired autophagy flux induced by oxLDL. (a) Autophagosomes in THP-1 cells detected by TEM. Autophagosomes are indicated by arrows. . (b, c) Representative western blot analysis of the protein expression levels of LC3II/LC3I and p62. All data are representative of three independent experiments. (d) Fluorescence microscopy images of GFP-RFP-LC3 adenovirus transfected THP-1 cells treated with oxLDL, oxLDL+ fucoidan, or 50 nM baf A1 for 36 h. . (e) The number of autolysosomes (red puncta) per cell was counted. Data are shown as the . vs. the untreated group; # vs. the oxLDL-treated group.
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