Oxidative Stress Induces Chondrocyte Apoptosis through Caspase-Dependent and Caspase-Independent Mitochondrial Pathways and the Antioxidant Mechanism of Angelica Sinensis Polysaccharide

Figure 3

The detection of oxidative stress in different groups. (a) Cultured and treated chondrocytes were harvested, and the levels of intracellular ROS in different groups were analyzed with a FACScan flow cytometer. (b) Cultured and treated chondrocytes were harvested, total proteins were extracted, and the levels of MDA equivalents were detected by assay kit. (c) Cultured and treated chondrocytes were harvested, total proteins were extracted, and the levels of CAT were detected by assay kit. Results are presented as of three independent experiments. , , versus normal control. ^#, ^## versus H₂O₂ group.

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