Research Article

Isokotomolide A from Cinnamomum kotoense Induce Melanoma Autophagy and Apoptosis In Vivo and In Vitro

Figure 3

Cell apoptotic death and cycle distribution in B16F10 cells of Iso A and Sec A. (a, b) Iso A and Sec A induced cell apoptotic death. Apoptosis was analyzed by a flow cytometry after exposed to Iso A from 0 to 100 μM for 24 h and stained with annexin V/PI. (c, d) Effects of Iso A and Sec A on the cell cycle distribution in B16F10 cells. The cell cycle distribution in Iso A-induced B16F10 cells was determined by the flow cytometry. Cells were administered from 0 to 100 μM for 24 h. The data represented of three independent experiments performed. .
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