Isokotomolide A from Cinnamomum kotoense Induce Melanoma Autophagy and Apoptosis In Vivo and In Vitro

Figure 4

The autophagy and apoptosis-related mRNA and proteins induced by Iso A and Sec A in B16F10 cells. (a) Atg 3, 6, and 12 mRNA expression in B16F10 cells treated with different concentration (25 and 100 μM) was evaluated by the quantitative polymerase chain reaction and normalized to the GAPDH gene. (b) The autophagic-related proteins Beclin 1, Atg 10, 5, and 16 expressions. β-Actin was viewed as an internal control. (c) The mRNA expression of caspase 9, Bax, and Bad in B16F10 cells treated with different concentrations (25 and 100 μM) was evaluated by the quantitative polymerase chain reaction and normalized to the GAPDH gene. (d) The apoptosis-related proteins JNK, Bcl-2, Cytochrome c, Caspase 9, and 3 expressions in B16F10 cells with or without Iso A and Sec A treatment. β-Actin was viewed as an internal control. The data represented of three independent experiments performed. and . .

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