Isokotomolide A from Cinnamomum kotoense Induce Melanoma Autophagy and Apoptosis In Vivo and In Vitro

Figure 6

Animal test and histopathologic analysis of melanoma tumor inhibition (induced by B16F10 cells) treated with Iso A. . (a) Antitumor activity of Iso A in nude mice xenograft model. Mice morbidity-free survival efficacy, tumor weight, and tumor volume measurements were demonstrated after sacrifices. (b) H&E staining of tumor mass of BALB/c mice in the dorsal implant with xenograft B16F10 melanoma tumor cells of Iso A treatment. Smaller tumor mass and tumor cells expressed round to polygonal shapes with several characteristics, including melanin pigment, high mitosis, and central massive necrosis in the mouse. (c) Apoptotic protein LC3B expression and autophagic protein cleavage caspase 3 secretion in tissues treated with/without Iso A detected by immunohistochemistry. The data represented of three independent experiments performed. and .

(a)

(b)

(c)