Shen-Hong-Tong-Luo Formula Attenuates Macrophage Inflammation and Lipid Accumulation through the Activation of the PPAR-γ/LXR-α/ABCA1 Pathway

Figure 7

SHTL inhibits LPS-induced inflammatory response in a PPAR-γ dependent manner in mouse peritoneal macrophages. (a) MPMs were incubated with SHTL (125 μg/mL) or GW9662 (PPAR-γ antagonist, 10 μM) for 1 h, then exposed to LPS (100 ng/mL) for 8 h. Intracellular ROS levels in MPMs were evaluated using flow cytometry. (b) Bar graph shows fluorescence intensity of ROS content in different groups from (a). (c, d) After the pretreatment with SHTL and/or GW9662 along with the incubation with LPS, the relative mRNA levels of TNF-α and IL-6 in MPMs and the content of TNF-α and IL-6 in the culture supernatants were detected by qPCR and ELSIA kits, respectively. and versus the Ctrl group; ^# and ^## versus the LPS group; ^&, ^&&, and ^&&& versus the SHTL group, .

(a)

(b)

(c)

(d)