Hydroxy-α-sanshool Possesses Protective Potentials on H2O2-Stimulated PC12 Cells by Suppression of Oxidative Stress-Induced Apoptosis through Regulation of PI3K/Akt Signal Pathway
Effects of HAS on protein expressions of caspase-3, Bax, Bcl-2, PI3K, p-PI3K, Akt, and p-Akt in H2O2-stimulated PC12 cells. PC12 cells were treated with HAS (15, 30, and 60 μM) or 100 μM vitamin C for 2 h, subsequently subjected to H2O2 (90 μM) for 4 h. Protein expressions of caspase-3, Bax, Bcl-2, PI3K, p-PI3K, Akt, and p-Akt were determined by western blotting, and β-actin was used as the internal reference. The values were represented as the (). vs. the control group.
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