Oxidative Medicine and Cellular Longevity / 2020 / Article / Fig 3

Research Article

Salidroside Ameliorates Mitochondria-Dependent Neuronal Apoptosis after Spinal Cord Ischemia-Reperfusion Injury Partially through Inhibiting Oxidative Stress and Promoting Mitophagy

Figure 3

Sal reduced the apoptosis of primary neurons and promoted neuronal survival after OGD/R in vitro. (a) The CCK-8 kit was used to detect the viability of neurons that were treated with different concentrations of Sal. (b) Brightfield images showing morphologic changes of primary neurons in control and OGD/R-treated samples in the presence or absence of different concentrations of Sal before OGD/R for 12 h. Scale bar, 100 μm. (c) Representative images of TUNEL and DAPI costaining of spinal cord neurons that were subjected to control or OGD/R for 12 h. Scale bar, 100 μm. (d) Quantification of apoptotic (TUNEL+) cells was performed (). (e) Examples of scatter plots for neurons that were subjected to control or OGD/R for 12 h by PI/Annexin V double labeling. (f) Quantification of apoptotic cells in (c). (g) Representative western blots of cleaved caspase-3/caspase-9, Bcl-2, and Bax expression in neurons that were subjected to control or OGD/R for 12 h. (h) Quantification of activated caspase-3/caspase-9 expression and the ratio of Bcl-2 to Bax normalized to the level in neurons that were treated with DMSO on the basis of the western blots previously described. β-Actin was used as loading control. All values in this figure were presented as (); , , and .
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