Oxidative Medicine and Cellular Longevity / 2020 / Article / Fig 4

Research Article

Salidroside Ameliorates Mitochondria-Dependent Neuronal Apoptosis after Spinal Cord Ischemia-Reperfusion Injury Partially through Inhibiting Oxidative Stress and Promoting Mitophagy

Figure 4

Sal inhibited oxidative stress and lessened the damage of reactive oxygen species to mitochondria. (a–d) SOD activity and MDA, GSSG, and GSH concentrations in the spinal cord were determined according to the respective assay kit results. (e) The levels of intracellular ROS in neurons were determined by a ROS assay kit. (f) Representative images of immunofluorescence of JC-1, indicative of . Scale bar, 100 μm. (g) Quantitative analysis of the change of the mitochondrial green/red fluorescence ratio. All values in this figure were presented as (); , , and .

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