Oxidative Medicine and Cellular Longevity / 2020 / Article / Fig 3

Research Article

SOCS2 Inhibits Mitochondrial Fatty Acid Oxidation via Suppressing LepR/JAK2/AMPK Signaling Pathway in Mouse Adipocytes

Figure 3

SOCS2 decreased mitochondrial fatty acid oxidation in mouse adipocytes. Four-week-old male mice were sacrificed, and stromal vascular cells were isolated from iWAT. (a) Protein expression level of SOCS2 after 48 h treatment with control vector, Ad-SOCS2, or sh-SOCS2 (); GAPDH was used as the loading control. (b) mRNA levels of SOCS1 and SOCS3 in iWAT after 48 h treatment with control vector, Ad-SOCS2, and sh-SOCS2 (). (c–j) Cells were transfected with different virus and induced to differentiation for 8 days and harvest for analysis (). (c) Bodipy staining; cells were carried out in mature adipocytes with respective treatment mentioned above. (d, e). Cellular TG content and FFA content quantification. (f, g) MCAD and LCAD protein levels. (h) Palmitate oxidation to CO2 was measured for 3 h (). (i) mRNA level of leptin receptor with qPCR. (j) Protein expression levels of CPT-1b, p-ACC, ACC1, p-JAK, and JAK; GAPDH was used as the loading control. Values are the . , .
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