Research Article

Fasting Induces Hepatocellular Carcinoma Cell Apoptosis by Inhibiting SET8 Expression

Figure 2

Effects of fasting on cell viability, apoptosis, ROS level, and expression of Keap1/Nrf2/ARE signalling pathway components and SET8. (a) Proliferation of MHCC-97H and HCC-LM3 cells in normal or fasting conditions for 24 h, as measured by CCK8. (b) Flow cytometry was used to detect apoptotic MHCC-97H and HCC-LM3 cells cultured in normal or fasting medium for 24 h. (c) The level of ROS was detected by flow cytometry in MHCC-97H and HCC-LM3 cells cultured in normal or fasting medium for 24 h. (d) Western blot analysis of the Keap1/Nrf2/ARE signalling pathway in MHCC-97H and HCC-LM3 cells cultured in normal or fasting conditions for 24 h. (e) The mRNA expression of the Keap1/Nrf2/ARE signalling pathway components in HCC cells grown in normal or fasting medium for 24 h was examined by qPCR. (f) Western blot analysis of SET8 and H4K20me1 in HCC cells cultured in normal or fasting medium for 24 h. (g) The mRNA expression of SET8 was examined by qPCR in cells grown in normal or fasting medium for 24 h. Data are shown as the of five independent experiments. vs. the control group.
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