Fasting Induces Hepatocellular Carcinoma Cell Apoptosis by Inhibiting SET8 Expression

Figure 3

Role of SET8 in HCC cell viability and apoptosis in response to fasting. (a) Proliferation of MHCC-97H and HCC-LM3 cells under fasting conditions for 24 h or fasting in combination with overexpressed SET8. (b) Flow cytometry was used to detect the number of apoptotic MHCC-97H and HCC-LM3 cells under fasting conditions for 24 h or fasting in combination with overexpressed SET8. (c) The level of reactive oxygen species in MHCC-97H and HCC-LM3 cells under fasting conditions for 24 h or fasting in combination with overexpressed SET8. (d) Western blot analysis of SET8 and Keap1/Nrf2/ARE signalling pathway components in MHCC-97H and HCC-LM3 cells under fasting conditions for 24 h or fasting in combination with overexpressed SET8. (e) qPCR analysis of SET8 and Keap1/Nrf2/ARE signalling pathway components in MHCC-97H and HCC-LM3 cells under fasting conditions for 24 h or fasting in combination with overexpressed SET8. (f) ChIP assay of H4K20me1 presence at the promoter region of Keap1. Normal IgG was used as a control. (g) SET8 knockdown increased Keap1 luciferase activity in HCC-LM3 cells. Data are shown as the of five independent experiments. vs. the control group. vs. the fasting group.

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