Research Article

Fasting Induces Hepatocellular Carcinoma Cell Apoptosis by Inhibiting SET8 Expression

Figure 6

SET8 interacts with PGC1α to regulate Keap1 in HCC cells in vitro, and fasting inhibits HCC growth via SET8 inhibition in vivo. (a) The effects of SET8, PGC1α, and mutant SET8 on Keap1 luciferase activity in HCC-LM3 cells. (b) Western blotting was used to detect the SET8, Keap1, and Nrf2 proteins in MHCC-97 and HCC-LM3 cells overexpressing wild-type or mutant SET8. (c, d) Tumour growth following subcutaneous injection in the right flanks of Balb/c nude mice with 97H control cells, 97H-shSET8 cells, or 97H-SET8 overexpression cells (). (e) Western blotting was used to detect proteins SET8 and Keap1/Nrf2/ARE signalling pathway components in mice. (f) The mRNA expression of SET8 and Keap1/Nrf2/ARE signalling pathway components was examined by qPCR in vivo. Data are shown as the of five independent experiments. vs. the control group. vs. the fasting group.
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