Oxidative Medicine and Cellular Longevity / 2020 / Article / Fig 4

Research Article

Pterostilbene Attenuates Cocultured BV-2 Microglial Inflammation-Mediated SH-SY5Y Neuronal Oxidative Injury via SIRT-1 Signalling

Figure 4

PTE promotes SIRT-1 expression, suppresses acetylation of p65 unit of NF-κB, and reduces inflammatory factor release in LPS-activated BV-2 cells, which can be abolished by EX527. BV-2 microglial cells were pretreated with vehicle or EX527 (100 nM) for 24 h and then incubated with vehicle or PTE (5.0 μM) for 2 h, followed by coculturing with SH-SY5Y cells with or without the presence of LPS (100 ng/mL) for 24 h. (a) The expression of SIRT-1 in BV-2 cells was measured using western blot analysis and normalized to that of β-actin. (b) The acetylated p65 (L310) in BV-2 cells was measured using western blot analysis and normalized to that of total p65. (c) The TNF-α and (d) IL-6 in supernatants were determined using an ELISA assay. Data are shown as . . α, compared with the control. β, compared with the a-BV-2. γ, compared with the PTE 2.5 μM. LPS: lipopolysaccharide; a-BV-2: LPS-activated BV-2 coculture group.
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