Pterostilbene Attenuates Cocultured BV-2 Microglial Inflammation-Mediated SH-SY5Y Neuronal Oxidative Injury via SIRT-1 Signalling

Figure 5

PTE attenuates apoptosis and oxidative stress of SH-SY5Y cells in the cocultured system with LPS-activated BV-2 cells, which can be abolished by EX527. BV-2 cells were pretreated with vehicle or EX527 (100 nM) for 24 h and then incubated with vehicle or PTE (5.0 μM) for 2 h, followed by coculturing with SH-SY5Y cells with or without the presence of LPS (100 ng/mL) for 24 h. (a) The expression of Bcl-2 and Bax in SH-SY5Y cells was measured using western blot analysis and was shown as the ratio of Bcl-2 to Bax. (b) The cleaved caspase 3 in SH-SY5Y cells was measured using western blot analysis and normalized to that of total caspase 3. (c) The level of ROS was measured using DCFH-DA staining and expressed as an average intracellular fluorescent density. (d) The MDA level, (d) SOD activity, and (e) GSH level in SH-SY5Y cells were measured using standardized commercial kits. Data are shown as . . ^α, compared with the control. ^β, compared with the a-BV-2. ^γ, compared with the PTE 2.5 μM. LPS: lipopolysaccharide; a-BV-2: LPS-activated BV-2 coculture group; ROS: reactive oxygen species; MDA, malondialdehyde: SOD, superoxide dismutase; GSH; glutathione.

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