Cerebral Mitochondrial Function and Cognitive Performance during Aging: A Longitudinal Study in NMRI Mice

Table 2

Relative normalized mRNA expression levels in brain homogenate from 3-, 6-, 12-, 18-, and 24-month-old mice determined using quantitative real-time PCR in comparison to 3-month-old control animals. mRNA expression of 3-month-old control mice is 100%; ; with one-way ANOVA and Tukey’s post hoc test with , , , and . “A” indicates one-way ANOVA and Tukey’s post hoc test against 6-month-old mice, “B” against 12-month-old mice, and “C” against 18-month-old animals. Results are normalized to the mRNA expression levels of beta 2 microglobulin (B2M) and phosphoglycerate kinase 1 (PGK1).

Gene	6 months	12 months	18 months	24 months
Complex I (CI)				^/AA/BBB/CC
Citrate synthase (CS)				^/A/B
Growth-associated protein (GAP43)				^/C
Synaptophysin 1 (SYP1)			^AA	^/B/CCCC
Superoxide dismutase 2 (SOD2)		^A	^/AAA	^/AAAA/B
Catalase (Cat1)			^/AAAA/B	^/AAAA/B
Peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC1-α)
Nuclear respiratory factor-1 (NRF-1)				^/AA/B/CC