Research Article

Anticancer Effects of Plasma-Activated Medium Produced by a Microwave-Excited Atmospheric Pressure Argon Plasma Jet

Figure 4

Measurement of H2O2 concentration in PAM. Amplex red hydrogen peroxide/peroxidase assay kit was used to measure H2O2 change in the PAM. The PAM was produced at the conditions; the gas flow rates 1.3 SLM and 1.9 SLM, the input power of 7 W, and the exposure times 60, 120, and 180 sec. H2O2 concentration was measured immediately and 0.5, 1, 2, 3, 6, 12, and 24 hours after plasma treatment on growth medium. Figures (a, b) show the H2O2 levels in the stored PAM. Figures (c, d) show the H2O2 concentration of treated PAM on A549 cells. After the cell treatment, the concentration of H2O2 in PAM was measured using Amplex red fluorescence ().
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