Anticancer Effects of Plasma-Activated Medium Produced by a Microwave-Excited Atmospheric Pressure Argon Plasma Jet

Figure 5

Detection of intracellular ROS in A549 and Nuff cells. (a) H₂O₂ concentration was measured 1, 2, 3, and 24 hours after incubation with PAM. Consumed NO₂^– or H₂O₂ concentration (μM) was calculated as concentration of storage PAM–concentration of incubated PAM with the cells. (b) NO₂^– levels were measured 1, 2, 3, and 24 hours after plasma treatment in the PAM incubated with cells. (c) Histogram showed the intracellular ROS generation after PAM treatment on A549 cells for 2 hours. (d) Bar graphs showed the quantitative analyses of the intracellular ROS after PAM treatment for 1, 2, 3, and 24 hours. Intracellular ROS of Nuff cells treated with PAM for 2 hours (e) and bar graphs (f). The plasma exposure conditions were two flow rates (1.3 SLM and 1.9 SLM), three exposure times (60, 120, and 180 sec), and input power 7 W. , , and vs. control. n.s. denotes vs. controls.

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