Age-Related Maintenance of the Autophagy-Lysosomal System Is Dependent on Skeletal Muscle Type

Figure 1

Posttranslational modifications in young and old SOL and EDL. All graphics show the comparisons between young SOL (white), young EDL (light grey), old SOL (grey), and old EDL (dark grey), by this order: (a) a representative immunoblot and quantification of 3-nitrotyrosine (3NT) protein against 3-nitrotyrosine (3NT) protein; (b) a representative immunoblot and quantification of pentosidine of pentosidine (AGE); (c) a representative immunoblot and quantification of K63-polyubiquitinated (K63) proteins. All blots with the respective densitometric quantifications are normalized with Ponceau S staining and further to the respective young muscle (results in percentage). For each analysis, 5-6 mice from each age group were used. Statistical significance was given as follows (Mann-Whitney test). Values are presented as

(a)

(b)

(c)