The HIPPO Transducer YAP and Its Targets CTGF and Cyr61 Drive a Paracrine Signalling in Cold Atmospheric Plasma-Mediated Wound Healing

Figure 5

Effect of NAC on CAP-mediated wound healing and metabolic activity: metabolic activity of HaCaTs, GM Fbs, and coculture after 24 hr incubation with 2.5 mM NAC alone or in combination with CAP-treated media (a). Relative wound density of HaCaTs after treatment with NAC alone or in combination with CAP-treated media at 6, 12, 18, and 24 hr (b). Relative wound density of coculture after treatment with NAC alone or in combination with CAP-treated media at 6, 12, 18, and 24 hr (c). Data are presented as from at least three independent experiments performed in triplicate (a–c). Statistical analysis was performed using either unpaired -test with Welch’s correction (a) or 2-way ANOVA (b, c).

(a)

(b)

(c)