The HIPPO Transducer YAP and Its Targets CTGF and Cyr61 Drive a Paracrine Signalling in Cold Atmospheric Plasma-Mediated Wound Healing

Figure 7

Effect of CAP on paracrine signalling between keratinocytes and fibroblasts: HaCaT cells were incubated with conditioned media harvested from untreated or 10 and 30 s CAP-treated GM Fbs. Relative wound density of HaCaT cells was monitored after 6, 12, 18, and 24 hr (a). Secreted CTGF and Cyr61 (pg/ml) was analysed by ELISA (b). Relative wound density in HaCaT cells was monitored after treatment with 10 and 50 ng/ml CTGF and 100 ng/ml and 1 μg/ml Cyr61 (c, d). Data are presented as from three (a, c, d) or two (b) independent experiments, and statistical analysis was performed using 1- (b) or 2-way ANOVA (a, c, d).

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