Diazoxide Protects against Myocardial Ischemia/Reperfusion Injury by Moderating ERS via Regulation of the miR-10a/IRE1 Pathway
Upregulation of miR-10a is involved in H/R-induced cardiomyocyte injury. (a) MTT assay was used to detect the cell viability of each group. (b) miR-10a expression in cardiomyocytes was assessed by qRT-PCR. (c) The transfection efficiency of the miR-10a mimic and miR-10a inhibitor was detected by qRT-PCR in cardiomyocytes. (d) The effect of the miR-10a mimic and miR-10a inhibitor on cell viability of each group was detected by MTT analysis. (e) The effect of the miR-10a mimic and miR-10a inhibitor on LDH release of each group was detected by LDH analysis. (f) Protein expressions of Bcl-2 and Bax were detected using Western blot. (g) Quantitative analysis of (f). Data were expressed as in three independent experiments. For (a) and (b), ## and ###, compared to the normal group; and , compared to the I/R group. For (c), , compared to the NC group. For (d)–(f), #, ##, and ###, compared to the NC group; , , and , compared to the miR-10a group.