Research Article

Diazoxide Protects against Myocardial Ischemia/Reperfusion Injury by Moderating ERS via Regulation of the miR-10a/IRE1 Pathway

Figure 4

IRE1 is the target gene of miR-10a in cardiomyocytes. (a) Schematic representation of the biotinylated miRNA pull-down method. (b) The relative mRNA expression of IRE1, XBP1, and GRP78 using the biotinylated miRNA pull-down method. (c, d) The effect of the miR-10a mimic and miR-10a inhibitor on protein expression of IRE1 was detected using Western blot. (e) The effect of the miR-10a mimic and miR-10a inhibitor on mRNA expression of IRE1 was detected using qPCR. (f) Mutant sequence of IRE1 mRNA 3UTR. (g) The effect of the miR-10a mimic on transcriptional activity of IRE1 using luciferase activity assay. Data were expressed as in three independent experiments. and , compared to the NC group.
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